Neonatal IBs in area CA3 are highly regular. A, A 500-sec-long extracellular record from a P3 slice bathed in control ACSF (Mg2+-free, with 5 μm gabazine), illustrating the high regularity of nIBs.B, A segment expanded from the record inA, illustrating that intercluster intervals (two-sided arrows) but not intracluster intervals were included in the analysis in this study. C, The IBI histogram for the experiment ofA shows two well separated peaks; the peak below 5 sec consists of intracluster intervals, which were excluded in the calculation of the CIH (solid line). CVIBI for this experiment was 0.12. D, Superimposed CIHs from all 22 slices recorded in control ACSF. Note the tight clustering and steep slope of 16 CIHs on the left side of the plot, illustrating the pacemaker-like character of nIBs. E, The mean IBI of all slices showed no change with postnatal age. F, In contrast, the CVIBI increased significantly between P1 and P6 (note the regression line).