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. 2019 Sep 12;14(9):e0222436. doi: 10.1371/journal.pone.0222436

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Fig 3 — (A) MAb 4C2 binds to HA with native conformation. A/New Caledonia/20/1999 (H1) HA-pseudoviruses (HAT was not included in HA-pseudoviruses production, and thus HA presented as HA0) were treated under different pH conditions, then immunoprecipitated with MAb 4C2 or Chessie 8, and analyzed by Western blotting with antiserum against H1 HA2. Input: 1/8 of total sample used for immunoprecipitation; MAb Chessie 8: Negative immunoprecipitation control. (B) Stem antibodies compete against MAb 4C2 binding to HA. MAb 4C2 binding to H5 HA was competed with HA head antibodies (VN2.4 and H17-L2) and stem antibodies (C179, FI6, and CR6261) in a competition ELISA. Data are shown as means and standard deviations.