Endocannabinoids increase microglial cell migration. a–f, In the lower chamber of the Boyden chamber assay, we added AEA (a), meth-AEA (mAEA; b), 2-AG (2AG;c), PEA (d), DEA (e), and HEA (f). BV-2 cell migration toward these ligands was quantified, and results are expressed as a percentage of basal BV-2 cell migration (i.e., vehicle = 0.1% DMSO; dashed line) measured in individual experiments. *p < 0.05; **p < 0.01; significantly different from basal BV-2 cell migration (ANOVA followed by Dunnett's post hoc test). Values are mean ± SEM of 9–45 independent quantifications of migration (i.e., 3–15 separate experiments performed in triplicate).