Table 2.

Endocannabinoid contents in primary cultures of mouse neurons and microglial cells under basal conditions

	Endocannabinoid content
	Neurons (pmol/mg protein)	Microglial cells (pmol/mg protein)
AEA	0.7  ± 0.2	13.6  ± 4.3
2-AG	0.4  ± 0.1	8.2  ± 2.0
HEA	0.2  ± 0.1	1.7  ± 0.4
DEA	0.4  ± 0.1	4.1  ± 1.1

Mouse neurons and microglial cells cultured in 100 mm dishes contained 4.15 ± 0.2 and 0.55 ± 0.04 mg of protein per dish, respectively (n = 14). Cells were analyzed under basal conditions (i.e., in cell culture media); endocannabinoids were extracted from cells plus adjacent media, purified by HPLC, and quantified by CI-GC/MS. Values are mean ± SEM of 14 independent quantifications of endocannabinoids, each performed on one 100 mm dish of cells.