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. 2003 Feb 15;23(4):1360–1371. doi: 10.1523/JNEUROSCI.23-04-01360.2003

Fig. 4.

Fig. 4.

SDF-1 induces translocation of phosphorylated CREB into the nuclei of sympathetic neurons. E8 sympathetic neurons were cultured in defined minimal medium for 6 hr (A1–A3) and then exposed to 100 ng/ml SDF-1 (B1–B3), SDF-1 plus 20 μm CXCR4 antagonist AMD3100 (C1–C3), SDF-1 plus 100 ng/ml pertussis toxin (D1–D3), or SDF-1 plus 200 nm PKA inhibitor PKI (E1–E3). After 30 min the cultures were fixed and stained for phosphorylated CREB (red) and Islet-1 (green). SDF-1 induces translocation of CREB into the nuclei of sympathetic neurons, and this translocation is blocked by all three pharmacological agents.