Fig. 1.

Rotenone and LPS induce synergistic degeneration of dopaminergic neurons. Primary rat neuron–glia cultures were treated with vehicle, indicated concentrations of rotenone or LPS alone, or combinations of indicated concentrations of rotenone and LPS. Eight days later, neurotoxicity was determined by [³H]DA uptake assay and quantification of TH-IR neurons after immunostaining of dopaminergic neurons with an anti-TH antibody. The results are the mean ± SEM of three experiments performed in triplicate.Rot, Rotenone. **p < 0.005 compared with the cultures treated with either rotenone or LPS alone.