Fig. 2.

Rotenone and LPS-induced neurotoxicity is preferential to dopaminergic neurons. Rat neuron–glia cultures were treated with vehicle, 0.5 nm rotenone, and/or 0.5 nm rotenone. Eight days later, cultures were subjected to [³H]DA or [³H]GABA uptake assay (A) or immunostaining with an anti-TH or anti-NeuN antibody followed by quantification of the immunostained neurons (B). The results are the mean ± SEM of three experiments performed in triplicate. R + L, Rotenone plus LPS. **p < 0.005 compared with the control cultures.