Fig. 5.

Putative synaptic contacts between posterior PH-SNs and APRs viewed at higher magnification. A, Seven sequential frames that spanned the volume of neuropil containing the putative synaptic contact marked with an asterisk in Figure 4. Numbers at the bottom right of each panel indicate the depth of the section compared with the first image in the series. Overlapping appositions (arrows) between the PH-SN (red) and APR (green), indicated by yellow pixels, occurred in four consecutive frames, indicating that this putative synaptic contact spanned a depth of ∼2.9 μm.B, One frame from a different preparation on day L3 in which the processes of a posterior PH-SN and an APR abutted with negligible overlap (arrow). The scale bar refers to allpanels.