Skip to main content
. 2003 Feb 15;23(4):1237–1245. doi: 10.1523/JNEUROSCI.23-04-01237.2003

Fig. 2.

Fig. 2.

Flavopiridol acts upstream of mitochondrial dysfunction in proteasome inhibitor-treated cortical neurons.A, Cortical neurons were treated with lactacystin (lact; 10 μm) in the presence or absence of flavopiridol (flavo; 1 μm) or BAF (100 nm) for the indicated times, fixed, and immunostained for anti-cytochrome c (cyt c) plus the nuclear dye Hoechst. ctl, Control.B, Neurons were treated and immunostained as inA, and the percentage of neurons that had lost punctate mitochondrial cytochrome c (Cyt c) staining (open circles/gray bars) or that had apoptotic nuclei (Apop. Nuc.; open squares/black bars) was determined. The data points are representative of two independent experiments. n.s., Not significant.Lact, lactacystin; Flav, flavopiridol. *p < 0.001 compared with control; **p < 0.01 compared with lactacystin alone;#p < 0.001 compared with lactacystin alone. C, Neurons were treated as indicated and were separated into heavy membrane fractions enriched in mitochondria. Proteins were separated by SDS-PAGE (12%), blotted to nitrocellulose membranes, and probed with mouse anti-cytochrome c(1:2000). The membranes were then stripped and reprobed with mouse anti-COXIV (1:2500). ActD, Actinomycin D.