Skip to main content
. 2003 Feb 15;23(4):1237–1245. doi: 10.1523/JNEUROSCI.23-04-01237.2003

Fig. 3.

Fig. 3.

Retinoblastoma protein becomes phosphorylated early after proteasomal inhibition in cortical neurons. Cortical neurons were treated with lactacystin (10 μm) and, at the indicated times, washed and solubilized in SDS sample buffer. The protein was separated by SDS-PAGE, blotted to nitrocellulose membranes, and probed with rabbit anti-phosphoRb (phosRb; Ser 795; 1:1000) or rabbit anti-native pRb (1:1000, B). The membranes were stripped and reprobed with rabbit anti-ERK2 (1:5000,A, C). D, The optical density (O.D.) of bands of phosphoRb relative to ERK from four independent experiments was quantified and plotted. *p < 0.05 compared with control; **p < 0.05 compared with lactacystin alone.Ctl, Control; Lact, Lactacystin;Flav, flavopiridol; ActD, Actinomycin D.