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. 2003 Feb 15;23(4):1424–1431. doi: 10.1523/JNEUROSCI.23-04-01424.2003

Fig. 3.

Fig. 3.

β-gal is expressed in motoneurons of the lumbar spinal cord. The sciatic nerve was cut, and the proximal stump was inserted into a small chamber filled with Adv.EFα-LacZ(1 × 109 infectious units). After 7 d, rats were perfusion-fixed with 4% PFA, cross sections were cut, and β-gal was identified by histochemical staining with X-gal. β-gal-positive neuronal processes are visible extending from transduced motoneurons (arrowheads) to regions close to the midline and central canal.