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. 2003 Feb 15;23(4):1424–1431. doi: 10.1523/JNEUROSCI.23-04-01424.2003

Fig. 5.

Fig. 5.

A, Photomicrograph at the level of the lesion site in the pyramids showing the extent of the lesioned CST. The nonlesioned CST is demarcated by the anterograde marker BDA stained with ABC reagent (Vector Laboratories) and DAB.B–E, Completeness of the CST lesion demonstrated by anterograde markers in the lumbar spinal cord of unlesioned (B, C) and lesioned (D, E) animals. The unlesioned CST was traced with BDA and visualized with Alexa Fluor 488-conjugated streptavidin; the lesioned CST was traced with Fluoro-ruby.B, Section from a normal rat (sham surgery) showing the unlesioned CST labeled with BDA. C, The same section asB, showing the CST positive for Fluoro-ruby.D, Section from an animal with a complete CSTL, showing the unlesioned side of CST positive for BDA. E, Same section as D showing absence of Fluoro-ruby in the lesioned CST, indicating that the CSTL was complete.