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Chronic morphine (Morph) and withdrawal selectively affect orexin (OX) neurons. Confocal microscopy shows double-label immunohistochemistry for orexin or MCH with β-gal (A) or c-Fos (C). The data were quantified and summarized for CRE-LacZ (B) and for c-Fos (D) (n = 6 per group). Nal, Naltrexone. *p < 0.05.
