Table 3.
Immunohistochemical assessment of structural markers and the neuropeptide CGRP in DRG cells after lysolecithin treatment
| Nerve | Treatment | Side | Number of positive cells per DRG | ||
|---|---|---|---|---|---|
| NF-200 | Peripherin | CGRP | |||
| Sciatic | Lysolecithin | Ipsi | 78.5 ± 3.2 | 129.7 ± 19.6 | 145.9 ± 19.7 |
| Lysolecithin | Contra | 77.3 ± 2.2 | 119.7 ± 4.6 | 154.5 ± 9.1 | |
| Sham | Ipsi | 68.1 ± 9.6 | 109.4 ± 9.6 | 149.6 ± 5.8 | |
| Sham | Contra | 73.4 ± 2.7 | 103.9 ± 1.9 | 129.9 ± 12.9 | |
| Saphenous | Lysolecithin | Ipsi | 67.1 ± 6.2 | 120.4 ± 11.6 | 152.8 ± 8.7 |
| Lysolecithin | Contra | 74.3 ± 13.9 | 124.7 ± 8.6 | 135.3 ± 7.4 | |
| Sham | Ipsi | 69.9 ± 9.7 | 119.5 ± 9.1 | 139.6 ± 5.8 | |
| Sham | Contra | 71.7 ± 9.6 | 121.7 ± 4.6 | 132.4 ± 9.6 | |
The expression of the neuronal structural markers peripherin and NF-200 and the peptide CGRP in DRG cells ipsilateral and contralateral to lysolecithin treatment of the saphenous or sciatic nerve on day 13 after treatment and from sham animals (n = 4 in each case). There was no difference in the number of cells expressing either structural marker after treatment with lysolecithin as determined by a Kruskal–Wallis one-way ANOVA on ranks or paired Student'st test. Data are presented as average number of immunopositive cells per DRG ± SEM. Ipsi, Ipsilateral; Contra, contralateral.