Fig. 1.

Schematic explanation of neuron–glial coculture setups used in this study. A, For a simple neuron–glial coculture setup the virus-infected glia were transplanted directly into naive (no contact with virus) mixed neuronal–glial cultures after 24 hr of transgene expression. B, Some experiments required a setup by which glia were separated physically from neurons (membrane-delimited coculture). This system consisted of naive cultures prepared in 24-well plates; infected glia were separated by a culture plate insert. Both cocultures were maintained for 24 hr and then exposed to 3 mm glutamate (Glu) for a further 24 hr, followed by quantitation of neuronal viability.