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. 2003 Apr 15;23(8):3394–3406. doi: 10.1523/JNEUROSCI.23-08-03394.2003

Fig. 4.

Fig. 4.

Nrf2 overexpression in a subpopulation of cells confers widespread neuronal protection from oxidative glutamate toxicity. A, Immunocytochemistry for eGFP (green, identifying infected cells) and NSE (red marker, a neuron-selective marker). Within a typical ad-GFP-infected culture the infected neurons (yellow, red + green), uninfected neurons (red), and infected glia (green) can be observed. B, Group data evaluating the vulnerability of infected neurons to oxidative glutamate toxicity. Data are expressed as a percentage of GFP+NSE+ cells (presumed infected neurons) in the indicated glutamate treatment group as compared with the ad-GFP control group. VE, Vitamin E (α-tocopherol), 100 μm.C, Viability of GFP+NSE− cells (presumed infected glia) present per image was not affected significantly with glutamate treatment.D, Uninfected neurons within cultures containing Nrf2-infected cells are more resistant to oxidative glutamate toxicity. Data represent the mean ± SEM number of cells counted over triplicate wells from at least three independent experiments; *p < 0.05, compared with the GFP control no-glutamate group.