Fig. 5.

Nrf2 overexpression in mixed immature cortical cultures protects neurons from H₂O₂-mediated toxicity, but not staurosporine-induced apoptosis. Ad-GFP- and ad-Nrf2-infected cultures were allowed to express transgenes for 48 hr before exposure to 0.3–30 μm H₂O₂ (A;n = 3) or 0.1–10 μm staurosporine (B; n = 4) for a further 24 hr. Cells were stained for NSE to evaluate neuronal viability. Data represent the mean ± SEM from the indicated number of experiments performed in quadruplicate; *p < 0.05, compared with ad-GFP-infected control.