Fig. 6.

A, RT-PCR analysis of total RNA from rat midbrain slices to determine the presence of a transcript for rat VR1. Lane 2 shows the band of the expected size (256 bp) obtained from rat VR1 cDNA transcript. Lane 3 shows the GAPDH transcript (470 bp). A 100 bp ladder is also shown on the left side of each gel. M, Size markers; lane 1, nonretrotranscribed total RNA. B, Binding of [³H]AEA to substantia nigra membranes. The binding of 200 nm [³H]AEA was measured in the absence or in the presence of the CB1 or CB2 receptor antagonists SR141716 or SR144528, of the VR1 antagonist capsazepine (CZP), or of cold AEA, each used at 1 μm (100% = 5300 ± 500 dpm). *p < 0.01 and **p < 0.05 versus controls.