Fig. 3.

Choline-activated currents from +/T mice (α7L250T) are larger, slower, and inhibited by MLA. A, Typical currents obtained from a VTA DA neuron. At the arrow, 10 mm choline is pressure puffed for 40 msec (once every 2 min). The current was inhibited by 5 nm MLA.B, Similar MLA-sensitive currents were observed in VTA non-DA neurons. C, Typical choline-activated, MLA-inhibited currents are shown from an SNc DA neuron. Three traces were averaged for each representative current. Calibration: 100 pA, 0.5 sec. D, Bar graphs representing average amplitudes of choline-evoked currents recorded from VTA DA, VTA non-DA, and SNc DA neurons of +/T mice. **p < 0.001 significantly different from VTA values by Student's t test.