Table 3. Surface plasmon resonance affinities.

Immobilized G6b-B receptor (standard configuration)
Ligand	G6b-B	Kon	Koff	KD (M)
Heparin	Monomer	1.12 ± 0.39×106	2.01 ± 0.54×10−3	2.00 ± 1.17×10−9
	Dimer	0.60 ± 0.56×106	3.16 ± 1.17×10−3	7.76 ± 5.30×10−9
Fractionated HS	Monomer	1.33 ± 0.01×105	9.99 ± 0.16×10−4	7.47 ± 0.17×10−9
	Dimer	1.20 ± 0.08×105	1.71 ± 1.11×10−3	14.0 ± 8.26×10−9
Perlecan	Monomer	1.94 ± 1.72×102	1.01 ± 0.37×10−4	7.32 ± 4.64×10−7
	Dimer	5.79 ± 6.94×103	2.28 ± 2.51×10−3	4.74 ± 1.34×10−7
dp12	Monomer	0.31 ± 0.27×106	2.39 ± 1.79×10−3	8.12 ± 1.22×10−9
	Dimer	2.50 ± 2.72×106	4.60 ± 5.01×10−3	1.84 ± 0.01×10−9
Immobilized ligand (reversed configuration)
Ligand	G6b-B	Kon	Koff	KD (M)
Heparin	Monomer	1.30 ± 0.29×105	8.85 ± 0.40×10−2	6.99 ± 1.25×10−7
	Dimer	3.28 ± 0.53×105	1.73 ± 0.04×10−3	5.33 ± 0.75×10−9
Fractionated HS	Monomer	9.22 ± 2.67×103	6.40 ± 0.33×10−3	7.31 ± 2.47×10−7
	Dimer	3.76 ± 4.69×104	4.58 ± 6.32×10−4	7.70 ± 7.21×10-9
Perlecan	Monomer	6.73 ± 3.38×103	1.28 ± 0.24×10−3	2.28 ± 1.51×10−7
	Dimer	4.90 ± 2.16×104	6.78 ± 2.57×10−4	1.41 ± 0.09×10−8

Values are means ± SD from two independent experiments.