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. Author manuscript; available in PMC: 2020 Sep 12.
Published in final edited form as: Chemistry. 2019 Aug 20;25(51):11842–11846. doi: 10.1002/chem.201902771

Figure 2.

Figure 2.

Response of [1-13C]Cys and [1-13C2]IDA to Zn2+ can be detected by several magnetic resonance mechanisms. (A) 13C NMR Spectra for 50 mM [1-13C]Cys with varying Zn2+ concentrations (B) Standard titration curve of [1-13C]Cys in response to various Zn2+ concentrations. The insert shows the titration curve at low Zn2+ equivalents. (C) Spectra for 50 mM [1-13C2]IDA with various Zn2+ concentrations highlighting formation of a Zn2+ bound species, and increased linewidth upon Zn2+ binding. * represents natural abundance citric acid peak (D) Standard titration curve of [1-13C2]IDA.