KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| APC anti-human CD317 (BST2, Tetherin) | Biolegend | Cat#348410: RRID: AB_2067121 |
| PerCP-Cy™5.5 Mouse Anti-Human CD4 | BD bioscience | Cat#552838; RRID: AB_394488 |
| V450 Mouse Anti-Human CD3 | BD bioscience | Cat#560351; RRID: AB_1645168 |
| PE Mouse Anti-Human HLA-ABC | BD bioscience | Cat#555553; RRID: AB_395936 |
| CD4 Monoclonal Antibody (S3.5), APC | Thermo Fisher | Cat#MHCD0405; RRID: AB_1473570 |
| PE Mouse Anti-Human CD3 | BD bioscience | Cat#555333; RRID: AB_395740 |
| Monoclonal Mouse Anti-Human HLA-ABC Antigen/RPE |
Dako | Cat#R7000; RRID: AB_11192092 |
| PE Mouse Anti-Human CD28 | BD bioscience | Cat#348047; RRID: AB_400368 |
| APC Mouse Anti-Human CD184 | BD bioscience | Cat#555976; RRID: AB_398616 |
| APC Mouse IgG1, κ Isotype Ctrl (FC) Antibody | Biolegend | Cat#400121; RRID: AB_326443 |
| PerCP-Cy™5.5 Mouse IgG1 κ Isotype Control | BD bioscience | Cat#552834; RRID: AB_394484 |
| V450 Mouse IgG1, κ Isotype Control | BD bioscience | Cat#560373; RRID: AB_1645606 |
| PE Mouse IgG1, κ Isotype Control | BD bioscience | Cat#555749; RRID: AB_396091 |
| Bacterial and Virus Strains | ||
| pCG-SIVsmm FWR1 nef H192D C-AU1 IRES EGFP | This Paper | N/A |
| pCG-SIVsmm FWR1 nef H196D C-AU1 IRES EGFP | This Paper | N/A |
| pCG-SIVsmm FWR1 nef H192D/H196D C-AU1 IRES EGFP | This Paper | N/A |
| pCG-SIVsmm FWR1 nef W203S C-AU1 IRES EGFP | This Paper | N/A |
| pCG-SIVsmm FWR1 nef H192D/H196D/W203S C-AU1 IRES EGFP | This Paper | N/A |
| pBR-NL43 Δvpu env* nef* IRES-eGFP | Wildum et al. 2006 | N/A |
| pBR-NL43 Δvpu env* IRES-eGFP | Wildum et al. 2006 | N/A |
| pBR-NL43 Δvpu env* IRES-eGFP_SIVsmmFwr1 nef | Wildum et al. 2006 | N/A |
| pBR-NL43 Δvpu env* IRES-eGFP_SIVsmmFwr1 nef H192D | This Paper | N/A |
| pBR-NL43 Δvpu env* IRES-eGFP_SIVsmmFwr1 nef H196D | This Paper | N/A |
| pBR-NL43 Δvpu env* IRES-eGFP_SIVsmmFwr1 nef H192D/H196D | This Paper | N/A |
| pBR-NL43 Δvpu env* IRES-eGFP_SIVsmmFwr1 nef W203S | This Paper | N/A |
| pBR-NL43 Δvpu env* IRES-eGFP_SIVsmmFwr1 nef H192D/H196D/W203S | This Paper | N/A |
| pBR-NL43 Δvpu env* IRES-eGFP SIVmac239 nef | Wildum et al. 2006 | N/A |
| pBR-SIVmac239 IRES-eGFP | Schindler et al. 2006 | N/A |
| pBR-SIVmac239 nef* IRES-eGFP | Schindler et al. 2006 | N/A |
| pBR-SIVmac239_IRES-eGFP nef H192D | This Paper | N/A |
| pBR-SIVmac239_IRES-eGFP nef H196D | This Paper | N/A |
| pBR-SIVmac239_IRES-eGFP nef H192D/H196D | This Paper | N/A |
| pBR-SIVmac239_IRES-eGFP nef W203S | This Paper | N/A |
| pBR-SIVmac239_IRES-eGFP nef H192D/H196D/W203S | This Paper | N/A |
| pHIT-G-VSV-G | Fouchier et al. 1997 | N/A |
| pBJ6 (empty) | Rosa et al. 2015 | N/A |
| pBJ6 SERINC5-HA | Rosa et al. 2015 | N/A |
| pCAGGS_HIV-1 M NL4–3 Env (137 FLAG-tag) | Braun et al. 2019 | N/A |
| pCG-HIV-1 NL4–3 nef 3* IRES-DsRed | Mack et al. 2017 | N/A |
| pCG-rhesus G tetherin DsRed | Mack et al. 2017 | N/A |
| pCG-SIVsmm1 tetherin Fit2 | This Paper | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| 2,2’-Dipyridyldisulfide (2-PDS) | Alfa Aesar | A11118, CAS: 2127-03-9 |
| AP-2 β2 1–591 rat | This Paper | P62944 |
| AP-2 σ2 Rat | This Paper | P62744 |
| AP-2 μ2 1–141 mouse | This Paper | Q3TWV4 |
| AP-2 α 1–621 rat | This Paper | Q66HM2 |
| AP-2 α 1–396 rat | This Paper | Q66HM2 |
| sooty mangabey tetherin 4–26 C2S SIVsmm Nef 1–251 C55A chimera | This Paper | C3VHQ4/DQ092758 |
| Nef C55A SIVsmm | This Paper | DQ092758 |
| tetherin 2–26 rhesus macaque | This Paper | G0WVJ8 |
| Deposited Data | ||
| AP2ΔμC:SMM tetherin-SIVsmm Nef EM map | This Paper | EMD-20217 |
| AP2ΔμC:SMM tetherin-SIVsmm Nef EM PDB | This Paper | 6OWT |
| Experimental Models: Cell Lines | ||
| Human: HEK293T cells | ATCC | Cat# CRL-3216; RRID: CVCL_0063 |
| Human: TZM-bl cells | NIH | Cat# 8129; RRID: CVCL_B478 |
| Oligonucleotides | ||
| Primers used to generate pCG IRES eGFP expression plasmids and HIV-1 IRES eGFP proviral constructs harboring SIVsmmFwr1 nef | See table S2 of this paper | N/A |
| Primers used to generate SIVmac239 IRES eGFP proviral constructs harboring mutations in nef | See table S3 of this paper | N/A |
| Recombinant DNA | ||
| Rat AP-2S1/Mm AP2M1/Rat AP2A1-TEVsite-GST/His-TEVsite-Rat AP2B1 in pST39 vector | This Paper | AP2ΔμC |
| AP2S1: N97C/C99S in AP2ΔμC in pST39 vector | This Paper | AP2ΔμCss |
| AP2S1: N97C/C99S in AP2ΔμC in pST39 vector | This Paper | AP2ΔμC-β D3A/S5A |
| AP2B1: K5D/K6E/F7D/pST39 in pST39 vector | This Paper | AP2ΔμC-β K5D/K6E/F7D |
| AP2B1: T8A/T9A in pST39 vector | This Paper | AP2ΔμC-β T8A/T9A |
| AP2B1: K11D/K12D in pST39 vector | This Paper | AP2ΔμC-β K11D/K12D |
| AP2B1: I15D/F16D in pST39 construct | This Paper | AP2ΔμC -β I15D/F16D |
| AP2B1: residue (1–13) truncation in pST39 construct | This Paper | AP2ΔμC-β Δ(1–13) |
| AP2B1: residue (1–23) truncation in pST39 construct | This Paper | AP2ΔμC-β Δ(1–23) |
| Rat AP2S1 (1–143)/GST-TEVsite-Rat AP2A1 (1–396) in pST39 construct | This Paper | GST-α-σ2 hemi |
| Rh Tetherin (2–26)-MBP in LIC 2BT vector | This Paper | His-rh Tetherin-MBP |
| Rh Tetherin (2–26) wild type in pGST2 vector (BamHI/XhoI) | This Paper | GST-rh Tetherin-WT |
| Rh Tetherin: Y6A/Y8A in pGST2 vector | This Paper | GST-rh Tetherin-Y6A/Y8A |
| Rh Tetherin: D15R/pGST2 in pGST2 vector | This Paper | GST-rh Tetherin-D15R |
| Rh Tetherin: I16E in pGST2 vector | This Paper | GST-rh Tetherin-I16E |
| Rh Tetherin: W17D in pGST2 vector | This Paper | GST-rh Tetherin-W17D |
| Rh Tetherin: K18D in pGST2 vector | This Paper | GST-rh Tetherin-K18D |
| Rh Tetherin: E19A in pGST2 vector | This Paper | GST-rh Tetherin-E19A |
| SIVsmm Nef-C55A in LIC 2BT vector | This Paper | His-SIVsmm Nef-WT |
| SIVsmm Nef: Y133D in LIC 2BT vector | This Paper | SIVsmm Nef-Y133D |
| SIVsmm Nef: V180D/S183Y in LIC 2BT vector | This Paper | SIVsmm Nef-V180D/S183Y |
| SIVsmm Nef: E185A/A186H in LIC 2BT vector | This Paper | SIVsmm Nef-E185A/A186H |
| SIVsmm Nef: Q187A/E188S in LIC 2BT vector | This Paper | SIVsmm Nef-Q187A/E188S |
| SIVsmm Nef: D189K in LIC 2BT vector | This Paper | SIVsmm Nef-D189K |
| SIVsmm Nef: E190K in LIC 2BT vector | This Paper | SIVsmm Nef-E190K |
| SIVsmm Nef: H192D in LIC 2BT vector | This Paper | SIVsmm Nef-H192D |
| SIVsmm Nef: L194A/V195A in LIC 2BT vector | This Paper | SIVsmm Nef-L194A/V195A |
| SIVsmm Nef: H196D in LIC 2BT vector | This Paper | SIVsmm Nef-H196D |
| SIVsmm Nef: Q199A in LIC 2BT vector | This Paper | SIVsmm Nef-Q199A |
| SIVsmm Nef: W203S in LIC 2BT vector | This Paper | SIVsmm Nef-W203S |
| SIVsmm Nef: D204S/P206A in LIC 2BT vector | This Paper | SIVsmm Nef-D204S/P206A |
| Smm tetherin (4–26)-C9S/C25S-10aa linker (GSDEASEGSG)-SIVsmm Nef (1–251)-C55A-6xHis in pMBP2 vector (BamHI/XhoI) | This Paper | smm tetherin-SIVsmm Nef-6His |
| smm tetherin-C2S-10aa linker-SIVsmm Nef-C55A-Δ(28–36)-6xHis in pMBP2 vector (BamHI/XhoI) | This Paper | smm tetherin-SIVsmm Nef-ΔL1 |
| 10aa linker (GSDEASEGSG)-SIVsmm Nef (1–251)-C55A-Δ(28–36)-6xHis in pMBP2 vector (BamHI/XhoI) | This Paper | 10link-SIVsmm Nef-ΔL1 |
| Software and Algorithms | ||
| MotionCor2–1.0.0 | Zheng et al., 2017 | http://msg.ucsf.edu/em/software/motioncor2.html |
| Gct-v1.06 | Zhang, 2016 | https://www.mrc-lmb.cam.ac.uk/kzhang/Gctf/ |
| CTFIND4.1.12 | Rohou and Grigorieff, 2015 | http://grigoriefflab.janelia.org/ctffind4 |
| Relion-3.06 | Zivanov et al., 2018 | https://github.com/3dem/relion |
| cryoSPARC v2.8.0 | Punjani et al., 2017 | https://cryosparc.com |
| UCSF Chimera1.13.1 | Pettersen et al., 2004 | https://www.cgl.ucsf.edu/chimera/download.html |
| PyEM | Daniel Asarnow, UCSF; Github | https://github.com/asarnow/pyem |
| PyEM support scripts | This Paper | https://github.com/simonfromm/miscEM |
| Coot 0.9 | Emsley et al., 2010 | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| Phenix 1.12 | Adams et al., 2010 | https://www.phenix-online.org/download/ |
| emRinger | Barad et al., 2015 | https://github.com/fraser-lab/EMRinger |
| MolProbity | Davis et al., 2007 | Phenix package |
| HDExaminer | Sierra Analytics, Modesto, CA | http://massspec.com |
| Proteome Discoverer 2.1 | Thermo Scientific, Waltham, MA | http://www.thermofisher.com/ |
| StatView version 4.0 | Abacus Concepts, Berkeley, CA | http://www.macintoshrepository.org/10824-statview-4-0 |
| Origin | OriginLab, Northampton, MA | http://www.originlab.com |