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. Author manuscript; available in PMC: 2020 Sep 13.
Published in final edited form as: Circ Res. 2019 Aug 20;125(7):699–706. doi: 10.1161/CIRCRESAHA.118.314416

FIGURE 1.

FIGURE 1.

Cardiac α1A-AR mRNA and receptor levels are normal in the knockin mouse (GqKI) with a 6-amino acid substitution in the 3rd intracellular loop of the α1A. A. Diagram of the mutation: CCPGCC replaces DSEQVT at amino acid 228. B. α1-AR subtype mRNA levels in AMVMs (AU/HKGs, arbitrary units relative to 2 house keeping genes). C. Saturation binding for total α1-ARs in WT and mutant heart. WT levels are identical to those reported previously (Rokosh et al., 2002; Myagmar et al., 2017).18,34 D. Competition binding to identify the α1A as the site with high 5-methylurapidil affinity, ~20% α1A in both WT and GqKI. Prism was used for nonlinear fitting in C and D. All Ns are hearts.