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. Author manuscript; available in PMC: 2020 Sep 12.
Published before final editing as: Dev Neurosci. 2019 Mar 12:1–15. doi: 10.1159/000497056

Figure 2. Impaired hippocampal expression of Calbi in posterior coronal sections following HI and lack of protection by TH at P18.

Figure 2.

Box and whiskers plot represent mean optical densities (OD) adjusted for background for Calb1 immunoreactivity in whole hippocampus (A1 and B1), CA field (A2 and B2), and DG (A3 and B3) in anterior and posterior, respectively. Boxes are limited by the 75th and 25th percentiles (interquartile range, IQR) and whiskers are limited by the last data point within 1.5 times the IQR from the median (continuous line inside the box). Analysis by Kruskal-Wallis ANOVA with Dunn-Bonferroni correction; *, adjusted p-value <0.05. Representative Calb1 IHC section hippocampus within posterior coronal brain sections of sham (B4), NT (B4’), and TH (B4”). Bar, 1000 μm. Two photomicrographs (4X) of the hippocampus (i and ii) within the posterior coronal section were used to compose the final figure (B4). CA, cornus ammonis; DG, Dentate gyrus; NT, normothermia; TH, therapeutic hypothermia.