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. 2019 Aug 28;48(7):685–689. doi: 10.1007/s00249-019-01392-1

Table 1.

Kinetic constants of the HIV-1 PR catalyzed hydrolysis of a peptide substrate without and with crowders

Crowding 0 g/L EG
100 g/L
EG
200 g/L
PEG600
100 g/L
PEG6000
100 g/L
Vmax (RFU/s) 66 ± 4 11 ± 1 16 ± 6 19 ± 5 13 ± 4
Km (µM) 25 ± 5 86 ± 6 498 ± 221 189 ± 70 158 ± 60