Table 1.
Kinetic constants of the HIV-1 PR catalyzed hydrolysis of a peptide substrate without and with crowders
| Crowding | 0 g/L | EG 100 g/L |
EG 200 g/L |
PEG600 100 g/L |
PEG6000 100 g/L |
|---|---|---|---|---|---|
| Vmax (RFU/s) | 66 ± 4 | 11 ± 1 | 16 ± 6 | 19 ± 5 | 13 ± 4 |
| Km (µM) | 25 ± 5 | 86 ± 6 | 498 ± 221 | 189 ± 70 | 158 ± 60 |