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. 2019 Sep 12;10(9):678. doi: 10.1038/s41419-019-1909-2

Fig. 8. Erythromycin reduces airway NETs and ameliorates emphysema in mice.

Fig. 8

a The extracellular DNA and concentrations of NE, MPO, and CITH3 in BALF supernatants of chronic cigarette smoke exposed mice (CS), cigarette smoke exposed mice treated with erythromycin (EM), and air controls (Air). Data are expressed as medians (n = 10). The comparisons were determined by Kruskal–Wallis one-way ANOVA on ranks. *P < 0.05, ***P < 0.001. NS not significant. b Fresh BALF of mice was centrifuged, and the cell pellets were washed twice and finally resuspended in PBS containing 10% fetal bovine serum. The neutrophils in BALF were identified to CD11b+Ly-6G+ cells by flow cytomertry.The gating strategy was shown. c The proportions of neutrophils in CD11b+ cells in BALF were detected by flow cytometry. Data are expressed as medians (n = 10). The comparisons were determined by Kruskal–Wallis one-way ANOVA on ranks. **P < 0.01. NS not significant. d The total cell count and neutrophils count in BALF. Data are expressed as medians (n = 10). The comparisons were determined by Kruskal–Wallis one-way ANOVA on ranks. *P < 0.05,**P < 0.01, ***P < 0.001. NS not significant. e Representative haematoxylin and eosin (H&E) sections of lung tissues from air exposed mice (Air), chronic cigarette smoke exposed mice (CS), and chronic cigarette smoke exposed mice treated with erythromycin (EM). Original magnification ×200. Scale bars = 50 µm. f The mean linear intercept (MLI) values in Air group, CS group and EM group was analysed by two independent investigators. Data are expressed as medians (n = 10). The comparisons were determined by Kruskal–Wallis one-way ANOVA on ranks. *P < 0.05, ***P < 0.001. NS not significant. g The correlations between extracellular DNA in BALF of cigarette smoke exposed mice and their MLI were determined by Spearman’s rank correlation coefficient