Table 3.

Brain pharmacokinetic studies of compound 9b and SAHA^a.

Compound	Route	Dose (mg/kg)	Brain concentration (ng/mL)		Plasma concentration (ng/mL)		Brain/plasma ratio
			0.5 h	1 h	0.5 h	1 h	0.5 h	1 h
9b	IV	2	414.6 ± 88.7	52.9 ± 1.6	46.0 ± 13.9	7.3 ± 1.0	9.0	7.2
SAHA	IV	2	14.5 ± 2.8	BQL	39.2 ± 11.5	4.5 ± 2.7	0.4	NA

^aCompound 9b and SAHA were administrated to ICR male mice by IV route at the dose of 2 mg/kg. Brain samples were collected at 0.5 and 1 hour time points and homogenized at a 1:4 ratio of tissue weight (g) to PBS volume (mL). Plasma samples were collected at 0.5 and 1 hour time points. Aliquots (20 μL) of brain homogenate or plasma samples were mixed with 180 μL of acetonitrile, vortexed, and centrifuged at 15,000 rpm for 5 minutes at 4 °C. The resulting supernatants were used for LC-MS/MS analysis. BQL: below quantifiable limit. NA: not applicable. Data are expressed as the mean ± SD (n = 4).