Fig. 1. Suppression of Bnip3 expression sensitizes BUMPT cells to OGD-R injury.

a Representative immunoblot of BNIP3. For OGD-R treatment, cells were subjected to 2 h OGD followed by 6 h reperfusion. Note: * indicated unspecific band. b Densitometry of BNIP3 signals in BUMPT cells with or without OGD-R treatment (n = 3). The BNIP3 signals were normalized to the β-actin signal of the same samples to determine the ratios. c Related Bnip3 mRNA levels in BUMPT cells stably expressing scrambled (Scr) shRNA or Bnip3-shRNA (n = 3). The Bnip3 mRNA levels were normalized to the Gaphd mRNA levels of the same sample to determine the rations. The ratios of control cells (Ctrl) were arbitrarily set as 1. d Representative images of TUNEL assay. Bar: 100 μm. e Apoptosis percentage (n = 3). f Representative immunoblots of active caspase-3. Cells were subjected to 2 h OGD followed by reperfusion for 10 min, 6 h, or 9 h, and whole-cell lysates were collected for immunoblot of activated caspase-3 and β-actin. d Densitometry of active caspase-3 (n = 3). The signal of cleaved caspase-3 was normalized to the β-actin signal of the same samples to determine the ratios. The ratios of control cells (Ctrl) were arbitrarily set as 1. Each symbol (circle and diamond) represents an independent experiment. Error bars: SEM. **p < 0.01