Fig. 5. Bnip3 deficiency enhances inflammation response in kidney following renal IR.

Bnip3-KO and WT mice were subjected to renal IR or sham operation (sham). Kidney tissues were collected for analysis of renal infiltration of macrophages and neutrophils, and for measurement of the mRNA levels of inflammatory cytokine IL-1b and TNF-α. a Representative images of macrophage and neutrophil staining. Bar: 50 μm. b Quantitation of macrophage-positive cells (n = 4). c Quantitation of neutrophil-positive cells. d Relative change of Il-1b transcript levels in kidney tissues (n = 3). e Relative change of Tnf-α transcript levels in kidney tissues (n = 3). The mRNA level of Il-1b and Tnf-α was normalized to the mRNA level of Gapdh in the same sample to determine the ratios, and the ratios of sham-operated WT were arbitrarily set as 1. Each symbol (circle and diamond) represents an individual mouse. Error bars: SEM. **p < 0.01; ***p < 0.001