Fig. 6. Bnip3 deficiency accumulates damaged mitochondria and ROS production following renal IR.

Bnip3-KO and WT mice were subjected to renal IR or sham operation (sham). Renal cortex was fixed and processed for TEM analysis and DHE staining. a Representative TEM images of mitochondrial morphology in proximal tubular cells. b Percentage of abnormal mitochondria (swollen with evidence of severely disrupted cristae over all mitochondria) (n = 4). c Representative images of DHE staining. DHE nuclear staining indicates the presence of reactive oxygen species (ROS) (n = 4). Scale bar: 100 μm. d Quantification of DHE fluorescence intensity. Each symbol (circle and diamond) represents an individual mouse. Error bars: SEM. **p < 0.01; ***p < 0.001