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. 2019 Sep 12;10(9):676. doi: 10.1038/s41419-019-1931-4

Fig. 1. Trehalose activates TFEB-mediated autophagy signaling in cultured SMCs.

Fig. 1

a, b SMCs were cultured in full-serum medium (10% FBS) and treated with trehalose (0–100 mM) for 24 h. Representative immunoblots and summarized data show the effects of trehalose on the protein expression levels of LC3-II and p62/SQSTM1. c–f SMCs treated with or without 100 mM trehalose cultured in full-serum medium or under starvation condition (Starv, 0.1%FBS) for 24 h. Representative immunofluorescence images (c) and quantification data (d) show the nuclear translocation of TFEB (green). Nuclei were stained with DAPI. e, f Real-time RT-PCR analyses of TFEB, LAMP-2A, LC3 and p62/SQSTM1 mRNA levels. g, h Immunoblots show the expression of TFEB in cytosolic or nuclear extract of SMCs (n = 4). i SMCs cultured in full-serum medium were treated with or without 100 mM trehalose in the absence or presence of bafilomycin (Baf, 50 nM) for 24 h. Representative immunoblots and summarized data show the protein expression levels of LC3-II. Scale bar = 50 µm. *P < 0.05 (n = 4)