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. 2019 Sep 12;10(9):676. doi: 10.1038/s41419-019-1931-4

Fig. 9. Trehalose attenuates proliferation and migration of SMCs and prevents HFD-induced neointima formation in PLCAs.

Fig. 9

The left carotid arteries of mice were partially ligated (ligation), and after ligation mice were fed HFD and treated with vehicle (PBS) or trehalose (i.p. 2 g/kg, every 2 days) for 4 weeks. a, b Representative IHC analysis of PCNA expression (brown color) in cross sections of PLCAs. c, d Representative immunofluorescence analysis of expression of MMP2 (red) and α-SMA (green) and their overlay (yellow) in cross sections of PLCAs. e, f Representative immunofluorescence analysis of expression of MMP9 (red) and α-SMA (green) and their overlay (yellow) in cross sections of PLCAs. g–i Representative H&E staining and quantification of intima area and intima-over-media ratio of cross sections of PLCAs. Scale bar = 100 μm. *P < 0.05 (n = 7–8)