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. 2019 Sep 12;10(9):681. doi: 10.1038/s41419-019-1928-z

Fig. 3. VEGF165 suppression leads to increased expression of α-SMA and collagen 1 and decreased expression of smad7 in endometrial stromal cells.

Fig. 3

a Human endometrial stromal cells were identified through morphological observation of the cells and through immunohistochemistry for cytokeratin 7 and vimentin. Scale bar = 100 μm. b The mRNA expression levels of VEGFA, collagen 1, and α-SMA in endometrial stromal cells were examined by qPCR at 6, 12, 24, and 48 h after transfection. N = 3, **P < 0.01, *P < 0.05. Unpaired t-test. c The protein levels of VEGFA, collagen 1, and α-SMA in endometrial stromal cells were examined by western blot analysis at 48 h after siVEGF165 transfection. N = 3, *P < 0.05. Unpaired t-test. d qPCR and western blot analysis confirmed that treatment with VEGF165 reversed the increased collagen 1 expression in endometrial stromal cells after VEGF165 suppression. N = 3, ***P < 0.001, **P < 0.01, *P < 0.05. Two-way ANOVA and SNK-q. e The mRNA expression of smad7 in human endometrial stromal cells was examined by qPCR at 6, 12, 24, and 48 h after transfection with siVEGF165. The protein levels of smad7 were examined by western blot analysis at 48 h after transfection with siVEGF165. N = 3, *P < 0.05. Unpaired t-test. siCTL siControl, siVEGF siVEGF165, VEGF VEGF165