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. 2019 Sep 12;10(9):681. doi: 10.1038/s41419-019-1928-z

Fig. 7. VEGF165 controls Notch signaling in endometrial stromal cells induced by TGFβ1.

Fig. 7

a The protein levels of DLL4, Notch1, Notch4, and Hes1 were examined by western blot analysis after ESCs were treated with 10 ng/mL TGFβ1 for 48 h and/or treated with 10 ng/mL VEGF165 for another 3, 6, 12, or 24 h. N = 3, ***P < 0.001, **P < 0.01, *P < 0.05. One-way ANOVA and SNK-q. b Representative images of DLL4, Notch1, Notch4, and Hes1 immunofluorescence staining in ESCs. Scale bar = 100 μm. N = 3, T: TGFβ1, VEGF: VEGF165. c Immunohistochemistry showed the expression patterns of Notch1 and Notch4 in human endometria at the late proliferative phase and in murine uteri in estrus. Scale bar = 100 μm. N = 3, **P < 0.01, *P < 0.05. Unpaired t-test