Fig. 7.

Cell and nuclear areas are both affected by perturbation to SUN2 levels. a Representative images of immortalized MSC nuclei stained against DAPI and SUN2 following control, SUN2 KD (siRNA2), OE and rescue (OE + siRNA2) treatments. Scatter plots showing log₂-fold change to b nuclear area, c cytoplasmic area, and d nuclear to cytoplasmic area ratio vs. log₂-fold change to SUN2 expression, in immortalized MSC nuclei (control, SUN2 KD, OE, and rescue), relative to control levels. Data points indicate individual nuclei (≥11 analysed per condition); annotated points show mean ± s.e.m. for each condition; lines show linear regression analysis (with annotation of gradient, b ± standard deviation). See Supplementary Fig. 10b–e for data distributions and statistics. Nuclear area showed weak positive power law scaling with SUN2 expression (b = 0.08 ± 0.04). However, as the cytoplasmic area showed stronger scaling with SUN2 expression (b = 0.38 ± 0.08), the nuclear to cytoplasmic area ratio had overall negative scaling (b = −0.38 ± 0.06). These results suggest a pronounced inside-to-outside influence of SUN2 levels on the cytoskeleton