Fig. 9.

γH2AX staining is increased in SUN2 OE hMSCs subjected to CTS. a The integrated intensity of γH2AX stained foci was significantly decreased in primary hMSCs immediately following CTS (p = 0.03) (2 h, 2.6–6.2% strain at 5 Hz; n = 4 donors). See Supplementary Fig. 12a for data distributions, donor-to-donor variation and representative images. Plot shows mean ± s.e.m. p-values were determined from linear modeling (ANOVA). b γH2AX staining was also significantly decreased in immortalised hMSCs 1 h after CTS (p = 0.0002) (1 h, 2.6–6.2% strain at 5 Hz; ≥ 29 cells analysed per condition, p-value from two-tailed t-test). c Quantification of γH2AX staining in immortalised hMSCs with inducible SUN2 OE, immediately following CTS (1 h, 2.6–6.2% strain at 5 Hz). CTS significantly increased DNA damage in cells with SUN2 OE (p < 0.0001), ≥27 nuclei analysed per condition. p-values determined by Dunnett’s multiple comparison tests. Box-whisker plot center lines show medians, bounds of box show quartiles, whiskers show data spread and outliers determined by the Tukey method. See Supplementary Table 1 for sample sizes