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. 2019 Sep 6;10:2121. doi: 10.3389/fimmu.2019.02121

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Copyright © 2019 Loyon, Jary, Salomé, Gomez-Cadena, Galaine, Kroemer, Romero, Trabanelli, Adotévi, Borg and Jandus.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Distribution of ILCs in healthy donors and in metastatic colorectal cancer patients. (A) Flow cytometry gating strategy for ILC subset identifications in PBMCs. (B) Frequencies of ILCtot. ILC subset distributions (C) and frequencies (D) were analyzed by flow cytometry in PBMCs of 51 healthy donors (HD) and 86 chemotherapy-naïve metastatic colorectal cancer patients (baseline). Distribution (E) and frequencies (F) of ILC subsets based on the frequency of ILCtot distributed in the low (n = 29), medium (n = 29), and high (n = 28) terciles. Columns, the means of ILC frequency for each patient; bars, SEM. *p < 0.05, **p < 0.005, ****p < 0.0001, as determined by Student's t-test or ANOVA.