Figure 1.

Morphodynamic Metrics used to Quantify Protrusive Behavior and Coiling Dynamics on Protrusive Assay

(A) Schematic showing the STEP protrusion platform (large-diameter base fibers deposited orthogonally onto smaller diameter protrusive fibers) along with metrics used to quantitate protrusive cycle and protrusion coiling. On left are data showing control on protrusive fiber diameter using STEP platform (n = 71, 51, 36, 30, and 40 for 135, 270, 450, 600, and 1,000 nm, respectively).

(B) Representative transient protrusive cycle showing protrusion growth and retraction to cell body.

(C) Representative images of protrusion coiling on the different fiber diameter categories studied. Left panels show scanning electron microscopic images of base-protrusive fiber combinations.

(D) Temporal evolution of protrusion coiling on a 600-nm-diameter fiber.

(E) Schematic showing the growth of the protrusion during a coiling cycle.

Scale bars, 5 μm. Data are represented as mean ± SEM.