Table 2.

Fold Change of Gene Targets in Primary Human Lung Cells Transfected with Synthetic Pollen miRNAs

Cells	pta-miR948			pta-miR1314
	Normal Epithelial	Asthmatic Epithelial	Endothelial (HMLEC)	Normal Epithelial	Asthmatic Epithelial	Endothelial (HMLEC)
Gene Target
HIVEP3	1.32 ± 0.2a	0.92 ± 0.3a	0.59 ± 0.001b	1.66 ± 0.4b	1.09 ± 0.2b	0.85 ± 0.1b
	p=0.03	p = n.s.	p=0.00005	p = n.s.	p = n.s.	p = n.s.
PIK3AP1	0.80 ± 0.4a	0.54 ± 0.3a	2.24 ± 3.6b	0.44 ± 0.03b	0.53 ± 0.07b	2.03 ± 4.9b
	p = n.s.	p=0.02	p = n.s.	p = n.s.	p = n.s.	p = n.s.
PRR11	1.07 ± 0.6b	1.39 ± 0.7b	1.84 ± 0.8b	0.72 ± 0.7b	1.46 ± 0.7b	1.75 ± 1.0b
	p = n.s.	p = n.s.	p = n.s.	p = n.s.	p = n.s.	p = n.s.
MMP24	1.29 ± 0.3b	0.64 ± 0.05b	0.75 ± 0.1b	0.89 ± 0.3a	1.40 ± 0.6a	1.08 ± 0.01b
	p = n.s.	p = n.s.	p=0.02	p = n.s.	p = n.s.	p = n.s.
PAD5	0.70 ± 0.2b	0.64 ± 0.1b	1.28 ± 0.1b	0.46 ± 0.3b	1.11 ± 0.4b	1.19 ± 1.2b
	p = n.s.	p = n.s.	p = n.s.	p= 0.02	p = n.s.	p = n.s.

Gene targets were chosen based on the highest scores predicted using http://mirdb.org/and the sequence of each pollen miRNA listed in http://mirbase.org.

HIVEP3-Human Immunodeficiency Virus Type 1 Enhancer Binding Protein; PIK3AP1-Phospho-inositide 3-Kinase Adaptor Protein 1; PRR11-poly(A) polymerase D5; MMP24-Matrix Metallopeptidase 24; PAD5-Proline Rich 11.

^aExpression at 6 h post transfection

^bExpression at 24 h post transfection.