Fig. 6.

CBD treatment increases the keratinocyte layer in the epidermis and the expression of HMOX1. (A) Haematoxylin-eosin staining of 5 μm paraffin-embedded sections were analysed by bright field microscopy. (B) Representative images of HMOX1 immunohistochemistry from mouse skin after 5 days of treatment with vehicle, CBD 0.1% or 1% (C) Quantification of HO-1 stained area in mouse epidermis. *P < 0.05, ***P < 0.001 compared with control. Scale bars: 200 (left) and 100 μm (right). n = 15 for vehicle treated samples and n = 12 for CBD treated samples.