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. 2019 Sep 6;10:2104. doi: 10.3389/fimmu.2019.02104

Figure 2.

Figure 2

Δrop5 increases antigen presentation by BMMΦs and BMDCs and decreased survival in BMMΦs. Antigen presentation in WT and Irgm1/m3−/− BMMΦs (A) and WT and Irgm1/m3−/− BMDCs (B) infected with wild type OVA expressing parasites (RH-OVA), Δrop5 knockout (Δrop5), and complemented Δrop5 strain (ROP5C). For statistical analysis B3Z activation was compared between RH OVA and the specified parasite strain for the infecting APC (i.e., WT or Irgm1/m3−/−). Quantification (C) and immunofluorescence (D) of representative images of Irgb6 (green) and GRA5 (red) localization on the PV in BMMΦs. Comparison of in vitro killing of PVs (E) by primed and unprimed BMMΦs as determined by plaque forming units (PFUs) at day 6 post infection. Quantification (F) and immunofluorescence (G) representative images of Irgb6 (green) and GRA5 (red) localization on the PV in BMDCs. Comparison of in vitro killing of PVs (H) by primed and unprimed BMDCs as determined by PFUs at day 6 post infection. Mean ± SEM. Mann-Whitney test. *P < 0.05, **P < 0.01, ****P < 0.0001.