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. 2019 Sep 6;10:2104. doi: 10.3389/fimmu.2019.02104

Figure 5.

Figure 5

Δgra2 increases antigen presentation in BMMΦs in the presence of GRA6. Antigen presentation in WT and Irgm1/m3−/− BMMΦs (A) or WT and Irgm1/m3−/− BMDCs (E) infected with wild type OVA expressing parasites (RH-OVA), Δgra2 knockout (Δgra2), wild-type complemented Δgra2::GRA2 strain (GRA2), Δgra2Δgra4 double knockout (Δgra2Δgra4), Δgra4 knockout (Δgra4), Δgra2Δgra6 double knockout (Δgra2Δgra6), Δgra6 knockout (Δgra6), Δgra12 knockout (Δgra12). For statistical analysis B3Z activation was compared between RH OVA and the specified parasite strain for the infecting APC (i.e., WT or Irgm1/m3−/−). Quantification (B) and immunofluorescence (C) representative images of Irgb6 (green) and GRA5 (red) localization on the PV in BMMΦs. Comparison of fold change in percent killing of PVs between knockout and RH-OVA (D) by primed and unprimed BMMΦs as determined by PFUs at day 6 post infection. Quantification (F) and immunofluorescence (G) of representative images showing Irgb6 (green) and GRA5 (red) localization on the PV in BMDCs. Mean ± SEM. Mann-Whitney test. *P < 0.05, **P < 0.01.