Table 5.
Concentration (μg/ml) |
|||||||||
---|---|---|---|---|---|---|---|---|---|
Wavelength/nm | Replicates | Blank | 200 | 100 | 50 | 25 | 12.5 | ||
Exp. 1 | A570 | Rep. 1 | 1.0656 | 1.0033 | 0.8548 | 1.0472 | 1.1885 | 1.2751 | |
Rep. 2 | 1.0504 | 0.8598 | 1.0182 | 1.1179 | 1.1709 | 1.2578 | |||
Rep. 3 | 0.9836 | 1.1382 | 1.3914 | 1.0965 | 1.2815 | 1.3069 | |||
Rep. 4 | 0.9948 | 0.9712 | 1.1796 | 0.9138 | 1.1196 | 1.2775 | |||
Mean Absorbance of Reps. | 1.0236 | 0.99312 | 1.111 | 1.04385 | 1.19013 | 1.27933 | |||
A600 | Rep. 1 | 0.6045 | 1.0854 | 0.8395 | 1.0163 | 0.9057 | 0.9813 | ||
Rep. 2 | 0.6152 | 0.8481 | 0.9751 | 0.8347 | 0.8893 | 0.9626 | |||
Rep. 3 | 0.6472 | 1.1427 | 1.4323 | 0.8202 | 0.9755 | 0.9999 | |||
Rep. 4 | 0.6218 | 1.0194 | 1.1857 | 1.003 | 0.8564 | 0.9837 | |||
Mean Absorbance of Reps. | 0.62217 | 1.0239 | 1.10815 | 0.91855 | 0.90673 | 0.98187 | |||
Net Absorbance (A570-A600) | 0.40143 | −0.0307 | 0.00285 | 0.1253 | 0.2834 | 0.29745 | |||
% Cell Viability | 100 | −7.6664 | 0.70997 | 31.2138 | 70.5985 | 74.0985 | |||
Exp. 2 | A570 | Rep. 1 | 0.9578 | 0.9334 | 0.8854 | 1.0416 | 1.1698 | 1.2876 | |
Rep. 2 | 1.1571 | 1.0754 | 1.0653 | 1.1424 | 1.1884 | 1.344 | |||
Rep. 3 | 1.1137 | 1.0846 | 1.4537 | 1.8403 | 1.1754 | 1.2491 | |||
Rep. 4 | 1.0617 | 0.9629 | 1.2382 | 1.0647 | 1.2895 | 1.34 | |||
Mean Absorbance of Reps. | 1.07257 | 1.01407 | 1.16065 | 1.27225 | 1.20578 | 1.30518 | |||
A600 | Rep. 1 | 0.5302 | 0.9295 | 0.8533 | 0.7479 | 0.9004 | 0.9852 | ||
Rep. 2 | 0.8297 | 0.8506 | 0.9948 | 0.8102 | 0.9322 | 1.0424 | |||
Rep. 3 | 0.764 | 1.1326 | 1.4649 | 1.7465 | 0.9557 | 0.9652 | |||
Rep. 4 | 0.83 | 0.9381 | 1.2156 | 1.0593 | 1.0168 | 1.0288 | |||
Mean Absorbance of Reps. | 0.73847 | 0.9627 | 1.13215 | 1.09097 | 0.95128 | 1.0054 | |||
Net Absorbance (A570-A600) | 0.3341 | 0.05138 | 0.0285 | 0.18128 | 0.2545 | 0.29978 | |||
% Cell Viability | 100 | 15.3771 | 8.53037 | 54.2577 | 76.1748 | 89.7261 | |||
Exp. 3 | A570 | Rep. 1 | 1.323 | 1.132 | 1.4486 | 1.2776 | 1.3187 | 1.4139 | |
Rep. 2 | 1.2966 | 1.2185 | 1.3799 | 1.2806 | 1.0512 | 1.374 | |||
Rep. 3 | 1.2871 | 1.1995 | 1.3893 | 1.2649 | 1.401 | 1.3359 | |||
Rep. 4 | 1.209 | 1.1804 | 1.35 | 1.111 | 1.2029 | 1.4066 | |||
Mean Absorbance of Reps. | 1.27892 | 1.1826 | 1.39195 | 1.23352 | 1.24345 | 1.3826 | |||
A600 | Rep. 1 | 1.0241 | 1.0888 | 1.0479 | 0.9571 | 1.1027 | 1.1306 | ||
Rep. 2 | 0.9343 | 1.0871 | 1.0662 | 0.9634 | 1.0164 | 1.0716 | |||
Rep. 3 | 0.9466 | 1.1122 | 0.9942 | 1.0648 | 1.1281 | 1.0312 | |||
Rep. 4 | 1.0884 | 1.2177 | 0.929 | 1.0298 | 0.9907 | 1.0983 | |||
Mean Absorbance of Reps. | 0.99835 | 1.12645 | 1.00932 | 1.00378 | 1.05948 | 1.08292 | |||
Net Absorbance (A570-A600) | 0.28057 | 0.05615 | 0.38263 | 0.22975 | 0.18398 | 0.29967 | |||
% Cell Viability | 100 | 20.0125 | 136.372 | 81.8854 | 65.5707 | 106.807 | |||
% Mean Cell Viability ± SD | 100 ± 0 | 9.24106 ± 7.16 | 48.5374 ± 76.16 | 55.7856 ± 25.37 | 70.7813 ± 5.3 | 90.2107 ± 16.36 |