Table 6.
Data for cytotoxicity of the AgNPs-L against PNT1A normal cells using the Resazurin metabolic assay.
| Concentration (μg/ml) |
||||||||
|---|---|---|---|---|---|---|---|---|
| Wavelength/nm | Replicates | Blank | 200 | 100 | 50 | 25 | 12.5 | |
| Exp. 1 | A570 | Rep. 1 | 1.2407 | 1.0866 | 1.2406 | 1.3137 | 1.2693 | 0.9282 |
| Rep. 2 | 1.1232 | 0.8972 | 1.28 | 1.2804 | 1.2856 | 0.9183 | ||
| Rep. 3 | 1.269 | 1.5388 | 1.1828 | 1.2567 | 1.069 | 0.9439 | ||
| Rep. 4 | 1.2601 | 1.2797 | 1.2009 | 1.303 | 1.235 | 0.8617 | ||
| Mean Absorbance of Reps. | 1.22325 | 1.20057 | 1.22607 | 1.28845 | 1.21472 | 0.91302 | ||
| A600 | Rep. 1 | 0.7743 | 0.746 | 0.9653 | 1.0537 | 0.9954 | 0.4771 | |
| Rep. 2 | 0.6654 | 0.9776 | 1.0465 | 1.0191 | 1.0113 | 1.0198 | ||
| Rep. 3 | 0.7117 | 0.95 | 0.9685 | 1.0086 | 0.8496 | 0.7099 | ||
| Rep. 4 | 0.9448 | 1.0352 | 0.9474 | 1.0497 | 0.9955 | 1.0908 | ||
| Mean Absorbance of Reps. | 0.77405 | 0.9272 | 0.98192 | 1.03277 | 0.96295 | 0.8244 | ||
| Net Absorbance (A570-A600) | 0.4492 | 0.27338 | 0.24415 | 0.25568 | 0.25177 | 0.08862 | ||
| % Cell Viability | 100 | 60.8582 | 54.3522 | 56.9179 | 56.0496 | 19.7295 | ||
| Exp. 2 | A570 | Rep. 1 | 1.1515 | 1.1619 | 1.1257 | 1.1935 | 1.1548 | 0.9842 |
| Rep. 2 | 1.211 | 1.3064 | 1.2002 | 1.1932 | 1.1526 | 1.3729 | ||
| Rep. 3 | 1.0892 | 1.382 | 1.4051 | 1.2978 | 1.2415 | 1.2624 | ||
| Rep. 4 | 1.2657 | 1.1699 | 1.1682 | 1.3704 | 1.2996 | 1.2985 | ||
| Mean Absorbance of Reps. | 1.17935 | 1.25505 | 1.2248 | 1.26372 | 1.21213 | 1.2295 | ||
| A600 | Rep. 1 | 0.5864 | 0.9968 | 0.8734 | 0.9459 | 0.9092 | 0.4771 | |
| Rep. 2 | 0.7193 | 0.9433 | 0.9986 | 0.9383 | 0.8934 | 1.0198 | ||
| Rep. 3 | 0.71 | 1.2182 | 1.1052 | 1.0181 | 0.9764 | 0.7099 | ||
| Rep. 4 | 0.8115 | 1.2238 | 0.9416 | 1.0979 | 1.0063 | 1.0908 | ||
| Mean Absorbance of Reps. | 0.7068 | 1.09552 | 0.9797 | 1.00005 | 0.94633 | 0.8244 | ||
| Net Absorbance (A570-A600) | 0.47255 | 0.15952 | 0.2451 | 0.26367 | 0.2658 | 0.4051 | ||
| % Cell Viability | 100 | 33.7583 | 51.8675 | 55.7983 | 56.248 | 85.7264 | ||
| Exp. 3 | A570 | Rep. 1 | 1.1275 | 1.035 | 1.2563 | 1.3947 | 1.2913 | 1.1149 |
| Rep. 2 | 1.2802 | 1.156 | 1.1997 | 1.4059 | 1.3251 | 1.246 | ||
| Rep. 3 | 1.2155 | 1.1991 | 1.2531 | 1.0914 | 1.3846 | 1.2462 | ||
| Rep. 4 | 1.2674 | 1.2178 | 1.2982 | 1.4632 | 1.3368 | 1.2599 | ||
| Mean Absorbance of Reps. | 1.22265 | 1.15198 | 1.25183 | 1.3388 | 1.33445 | 1.21675 | ||
| A600 | Rep. 1 | 0.8274 | 0.746 | 0.9999 | 1.1287 | 1.0471 | 0.9102 | |
| Rep. 2 | 0.6811 | 0.9776 | 0.979 | 1.1563 | 1.0674 | 0.623 | ||
| Rep. 3 | 0.6737 | 0.95 | 1.0378 | 0.8926 | 1.0979 | 0.9252 | ||
| Rep. 4 | 0.7668 | 1.0352 | 1.0329 | 1.1917 | 1.0738 | 0.9023 | ||
| Mean Absorbance of Reps. | 0.73725 | 0.9272 | 1.0124 | 1.09232 | 1.07155 | 0.84018 | ||
| Net Absorbance (A570-A600) | 0.4854 | 0.22478 | 0.23943 | 0.24648 | 0.2629 | 0.37657 | ||
| % Cell Viability | 100 | 46.3072 | 49.3253 | 50.7777 | 54.1615 | 77.5803 | ||
| % Mean Cell Viability ± SDs | 100 ± 0 | 46.9746 ± 13.56 | 51.8483 ± 2.51 | 54.498 ± 3.23 | 55.4864 ± 1.15 | 61.0121 ± 35.98 | ||