Table 7.
Data for cytotoxicity of 5FU against HeLa Cells using the Resazurin metabolic assay.
| Concentration (μg/ml) |
||||||||
|---|---|---|---|---|---|---|---|---|
| Wavelength/nm | Replicates | Blank | 200 | 100 | 50 | 25 | 12.5 | |
| Exp. 1 | A570 | Rep. 1 | 0.9300 | 0.9917 | 1.2199 | 0.8006 | 1.0193 | 1.0169 |
| Rep. 2 | 1.3646 | 1.186 | 1.2638 | 1.1729 | 1.1181 | 1.207 | ||
| Rep. 3 | 1.2094 | 1.0287 | 1.1894 | 1.1752 | 1.0646 | 1.1992 | ||
| Rep. 4 | 1.3059 | 1.171 | 1.1563 | 1.0809 | 1.1307 | 1.1797 | ||
| Mean Absorbance of Reps. | 1.20247 | 1.09435 | 1.20735 | 1.0574 | 1.08318 | 1.1507 | ||
| A600 | Rep. 1 | 0.6142 | 0.8624 | 1.127 | 0.699 | 0.9253 | 0.8917 | |
| Rep. 2 | 0.9294 | 1.1043 | 1.1901 | 1.0642 | 1.074 | 1.1148 | ||
| Rep. 3 | 1.0003 | 1.0022 | 1.1349 | 1.1609 | 1.0259 | 1.0741 | ||
| Rep. 4 | 0.8611 | 1.144 | 1.102 | 1.0754 | 1.0492 | 1.1133 | ||
| Mean Absorbance of Reps. | 0.85125 | 1.02823 | 1.1385 | 0.99988 | 1.0186 | 1.04847 | ||
| Net Absorbance (A570-A600) | 0.35122 | 0.06612 | 0.06885 | 0.05752 | 0.06457 | 0.10223 | ||
| % Cell Viability | 100 | 18.827 | 19.6028 | 16.3784 | 18.3856 | 29.1053 | ||
| Exp. 2 | A570 | Rep. 1 | 0.9551 | 1.017 | 1.2367 | 0.8372 | 1.0507 | 1.0592 |
| Rep. 2 | 1.4089 | 1.2117 | 1.3115 | 1.2111 | 1.1562 | 1.2348 | ||
| Rep. 3 | 1.2444 | 1.0983 | 1.2719 | 1.2354 | 1.12 | 1.2559 | ||
| Rep. 4 | 1.3292 | 1.2225 | 1.213 | 1.1333 | 1.1685 | 1.2467 | ||
| Mean Absorbance of Reps. | 1.2344 | 1.13737 | 1.25828 | 1.10425 | 1.12385 | 1.19915 | ||
| A600 | Rep. 1 | 0.6179 | 0.8865 | 1.1511 | 0.7214 | 0.9486 | 0.9195 | |
| Rep. 2 | 0.9504 | 1.1268 | 1.2173 | 1.0868 | 1.0954 | 1.1367 | ||
| Rep. 3 | 1.0168 | 1.0383 | 1.1735 | 1.2021 | 1.0573 | 1.1011 | ||
| Rep. 4 | 0.8705 | 1.1749 | 1.1333 | 1.1027 | 1.0712 | 1.1387 | ||
| Mean Absorbance of Reps. | 0.8639 | 1.05663 | 1.1688 | 1.02825 | 1.04312 | 1.074 | ||
| Net Absorbance (A570-A600) | 0.3705 | 0.08075 | 0.08948 | 0.076 | 0.08072 | 0.12515 | ||
| % Cell Viability | 100 | 21.7949 | 24.1498 | 20.5128 | 21.7881 | 33.7787 | ||
| Exp. 3 | A570 | Rep. 1 | 1.618 | 1.3869 | 1.4109 | 1.4461 | 1.5886 | 1.51 |
| Rep. 2 | 1.6132 | 1.2718 | 1.4172 | 1.5096 | 1.4624 | 1.5024 | ||
| Rep. 3 | 1.5871 | 1.306 | 1.4215 | 1.5138 | 1.374 | 1.4649 | ||
| Rep. 4 | 2.2674 | 1.2012 | 1.393 | 1.3669 | 1.3833 | 1.4478 | ||
| Mean Absorbance of Reps. | 1.77143 | 1.29147 | 1.41065 | 1.4591 | 1.45207 | 1.48128 | ||
| A600 | Rep. 1 | 0.7961 | 0.9932 | 0.9462 | 1.0964 | 0.7275 | 0.9649 | |
| Rep. 2 | 0.8412 | 0.8292 | 1.0772 | 1.0013 | 0.9073 | 0.8436 | ||
| Rep. 3 | 0.813 | 0.9733 | 1.0002 | 0.8611 | 1.0423 | 0.9563 | ||
| Rep. 4 | 1.0122 | 0.9924 | 1.1114 | 0.9739 | 0.9566 | 0.9414 | ||
| Mean Absorbance of Reps. | 0.86563 | 0.94703 | 1.03375 | 0.98318 | 0.90843 | 0.92655 | ||
| Net Absorbance (A570-A600) | 0.9058 | 0.34445 | 0.3769 | 0.47592 | 0.54365 | 0.55473 | ||
| % Cell Viability | 100 | 38.0272 | 41.6096 | 52.542 | 60.0188 | 61.2414 | ||
| Mean % Cell Viability ± SD | 100 ± 0 | 26.2163 ± 10.33 | 28.4541 ± 11.62 | 29.811 ± 19.79 | 33.3975 ± 23.11 | 41.3751 ± 17.36 | ||