Figure S8. Loss of Coa6 results in complex IV deficiency.
(A) Cells were solubilized in digitonin and subjected to BN-PAGE and immunoblot analysis using a total OXPHOS antibody cocktail. SC, supercomplexes consisting of CI, CIII, and CIV. (B) Unlike WTCoa6, the C58S/C90SCoa6-mutant localizes in the cytosol. The FLAG-tagged WTCoa6 and FLAG-tagged C58S/C90SCoa6 double mutant were expressed in COA6KO cells. Immunofluorescence was performed using Flag and Tom20 antibodies. The resulting images were merged using ImageJ software. The scale bar represents 10 μm.