Skip to main content
. 2019 Sep 12;2(5):e201900458. doi: 10.26508/lsa.201900458

Figure S8. Loss of Coa6 results in complex IV deficiency.

Figure S8.

(A) Cells were solubilized in digitonin and subjected to BN-PAGE and immunoblot analysis using a total OXPHOS antibody cocktail. SC, supercomplexes consisting of CI, CIII, and CIV. (B) Unlike WTCoa6, the C58S/C90SCoa6-mutant localizes in the cytosol. The FLAG-tagged WTCoa6 and FLAG-tagged C58S/C90SCoa6 double mutant were expressed in COA6KO cells. Immunofluorescence was performed using Flag and Tom20 antibodies. The resulting images were merged using ImageJ software. The scale bar represents 10 μm.