Fig. 1.

LTC treatment of dog leukocytes stimulates release of IFNγ and TNFα in a dose-dependent manner. Whole blood was obtained from healthy, purpose bred dogs (n = 3) and processed to generate peripheral blood mononuclear cells (PBMC) via density centrifugation through Ficoll. Cells were seeded in triplicate wells in complete tissue culture medium, as noted in Methods. The cells were then treated with the indicated amounts of LTC to activate cells and trigger cytokine production, which was monitored using specific canine ELISA assays for IFNγ and TNFα. Data were analyzed using one-way ANOVA with multiple comparisons. (*, P ≤ 0.05, **, P ≤ 0.01 ****, P ≤ 0.001). These results are representative of a total of 3 separate and independent experiments