Figure 2.
Effects of N- and C-terminally truncated GIP peptides alone or in combination with hGIP(1-42) (10−7 M) on insulin release from BRIN-BD11 cells.
BRIN-BD11 cells were incubated (20 min) with test peptides (10−12 to 10−6 M) alone or in combination with hGIP(1-42) (10−7 M) in the presence of 5.6 mM glucose. Insulin was measured by RIA. Values are mean ± standard error of mean (n = 8) for insulin release.
Abbreviations: GIP indicates glucose-dependent insulinotropic polypeptide; hGIP, human glucose-dependent insulinotropic polypeptide; mGIP, mouse glucose-dependent insulinotropic polypeptide.
***P < 0.001 compared with relevant glucose alone. ∆∆P < 0.01, ∆∆∆P < 0.001 compared with hGIP(1-42).