Skip to main content
. 2019 Aug 22;141(36):14026–14031. doi: 10.1021/jacs.9b03107

Figure 3.

Figure 3

(a) Model of the DFT optimized structure of (AX)3-(BX)3 at (B3LYP/6-31G(d) theory level (see Supporting Information). (b) Displacement reaction on a fluorescein-(AX)3 template: Monovalent (BX)1 was titrated to achieve maximum binding (fluorescence, blue) followed by the addition of increasing amounts of Dylight650-(BX)3 to displace (BX)1 (FRET, black). (c) Sequence dependent discrimination between complementary AAYA-BYBB and mismatching (AX)3-BYBB peptide based on titration. (d) Quantification of binding of PEG5000-(AX)3 with CytC-(BX)3 at 100 μM using Alizarin Red S assay. (e) Characterization of the recognition by CytC-(BX)3 (13 886 g mol–1) with PEG5000-(AX)3 (6468 g mol–1) yielding PEG5000-(AX)3(BX)3-CytC (calcd. MW: 20 036 g mol–1, detected MW 20 243 g mol–1, matrix CHCA) using MALDI-TOF MS.