Table 2.
Primer sequences and source of RT-PCR.
| gene | Primer (5′-3′) | GenBank No | Product size (bp) |
|---|---|---|---|
| 16SrRNA | 16S rRNA-F:GCTGCCCTTTGTATTGTC | CP012692.1 | 179 |
| 16S rRNA-R:AGATGTTGGGTTAAGTCCC | |||
| coa | coa-F:AAAGTTGGAAACCAGCAAGAGG | AB436977.1 | 98 |
| coa-R:GTGCCCTGTGGAATTTTAACTAATG | |||
| sarA | sarA-F: TGTTTGCTTCAGTGATTCGTTTA | LT671859.1 | 168 |
| sarA-R:AACCACAAGTTGTTAAAGCAGTTA | |||
| icaA | icaA-F:TGGGATACTGACATGATTACTGAGG | KF972125.1 | 109 |
| icaA-R:CAGGCACTAACATCCAGCATAGAG | |||
| cidA | cidA-F: ATTCATAAGCGTCTACACCTT | LT671859.1 | 178 |
| cidA-R:TTCTTCATACCGTCAGTTGT | |||
| sea | sea-F:TTGGAAACGGTTAAAACGAA | LC032460.1 | 121 |
| sea-R:GAACCTTCCCATCAAAAACA | |||
| seb | seb-F:TGTTCGGGTATTTGAAGATGG | CP013182.1 | 154 |
| seb-R:CGTTTCATAAGGCGAGTTGTT | |||
| sec | sec-F:GACATAAAAGCTAGGAATTT | CP013955.1 | 257 |
| sec-R:AAATCGGATTAACATTATCC | |||
| sed | sed-F:CCGTACAAGAATTAGATGC | CP007455.1 | 166 |
| sed-R:GGAAAATCACCCTTAACAT | |||
| see | see-F:TAATAACCGATTGACCGAAG | M21319.1 | 277 |
| see-R:ATCTGGATATTGCCCTTGAG | |||
| tsst-1 | tst-F:ACCCCTGTTCCCTTATCATC | CP001996.1 | 108 |
| tst-R:AAAAGCGTCAGACCCACTAC |